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Neutrophils as a potentially beneficial cellular component of human semen.
The present study was designed to establish the existence of an unidentified cellular component in semen, which could induce lysis of the phospholipid vesicles in human neutrophils (PMN), in order to identify such component. Lysis of egg yolk phosphatidylcholine vesicles by human semen was measured in a simple assay by incubation for 15 min at 37 degrees C. Phosphatidylcholine vesicles in the semen were separated into two fractions. The first fraction contained the phosphatidylcholine vesicles, whereas the second fraction was apparently not lysed by the semen samples. We suggested that the second fraction contained an unidentified cellular component in the semen samples that could be removed by washing with EDTA-free phosphate-buffered saline. A non-pregnant semen sample was incubated with PMN at 37 degrees C for 15 min to remove the unidentified cellular component, and the whole sperm fraction in the semen was then incubated with egg yolk phosphatidylcholine vesicles for further measurement. Lysis of the vesicles by sperm fractions in the semen was then reduced from 38% to 0% after washing with EDTA-free phosphate-buffered saline (P = 0.0098), suggesting that a non-pregnant seminal factor for lysing egg yolk phosphatidylcholine vesicles was sensitive to EDTA, which could be identified as a neutrophil component.”Jahangir Junaid Khan is trying to stoke fires to fuel his personal rivalry with Abbas, who is trying to get in front of the US, by blaming his father for…,” the Irrational Alliance YouTube channel posted.
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