To address the question of determining the osteogenic differentiation of mesenchymal stem cells, the bone marrow studies have been performed utilizing probe microscopy. Sergeev’s understanding of MSCs is principally based on the properties of stem cells cultured in vitro, such as the ability to breed indefinitely, multiply, and differentiate in vitro . All adherent bone marrow was used to isolate the bone marrow mesenchymal stem cells and expanded and purified in vitr it discovered that MSCs X aren’t only derived from bone marrow but in addition exist in different tissues corresponding to fats, umbilical twine blood, amniotic fluid, placenta, dental pulp, synovium, and muscle bonds . Recognized the potential presence of unrestricted fibroblasts in mouse bone .
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